Article Sidebar

Download
Statistic
Read Counter : 0

Downloads

Download data is not yet available.

Main Article Content

Abstract

This study aims to isolate collagen from the scales of snakehead fish (Channa sp.) and evaluate the antioxidant activity of the extracted collagen. Collagen was isolated using a 0.5 M acetic acid method, involving dissolution and purification procedures. The collagen extract was characterized using FTIR analysis to detect functional groups and phytochemical tests to identify active compounds. Antioxidant activity was assessed using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method to measure free radical scavenging potential. The results indicated that the collagen extract from snakehead fish scales contains bioactive compounds with significant antioxidant activity. The IC₅₀ values demonstrated a strong ability to neutralize free radicals, showing higher potency than the control. FTIR analysis detected hydroxyl (3330.21 cm⁻¹), methyl (2111.83 cm⁻¹), and carbonyl (1635.85 cm⁻¹) groups as characteristic phytochemical components of Channa sp. collagen extract. The IC₅₀ values at different concentrations were classified as very strong at low concentrations (25%), strong at moderate concentrations (50%), and moderate at higher concentrations (75%). The antioxidant activity of snakehead fish scale extract was very strong at lower concentrations but decreased as the concentration increased

Keywords

Antioxidant snakehead fish scales DPPH

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Author Biographies

Nily Su'aida, Program Studi Farmasi, Fakultas Farmasi, Universitas Islam Kalimantan Muhammad Arsyad Al-Banjari, Banjarmasin,

Program Studi Farmasi, Fakultas Farmasi, Universitas Islam Kalimantan Muhammad Arsyad Al-Banjari, Banjarmasin,

ainur Ridha, Program Studi Farmasi, Fakultas Farmasi, Universitas Islam Kalimantan Muhammad Arsyad Al-Banjari, Banjarmasin,

Program Studi Farmasi, Fakultas Farmasi, Universitas Islam Kalimantan Muhammad Arsyad Al-Banjari, Banjarmasin,

Yulistia Budianti Soemarie, Program Studi Farmasi, Fakultas Farmasi, Universitas Islam Kalimantan Muhammad Arsyad Al-Banjari, Banjarmasin,

Program Studi Farmasi, Fakultas Farmasi, Universitas Islam Kalimantan Muhammad Arsyad Al-Banjari, Banjarmasin,
How to Cite
Su’aida, N., Ridha, ainur, & Soemarie, Y. B. (2025). Karakteristik dan Uji Aktivitas Antiosidan Ekstrak Kolagen Sisik Ikan Gabus (Channa sp.). Borneo Journal of Pharmascientech, 9(2). https://doi.org/10.59053/bjp.v9i2.652
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Budianto, E., Yusuf, M., & Rahayu, D. (2021). Optimization of Fish Collagen Extraction and Its Application for Topical Formulations. Journal of Cosmetic Science, 72(2), 121-130. https://doi.org/10.1016/j.cosmet.2020.12.010
  2. Chen, J., et al. (2021). Extraction Temperature Is A Decisive Factor For The Properties Of Pectin. Food Hydrocolloids, 112, 106160. https://doi.org/10.1016/j.foodhyd.2020.106160
  3. Dorman, H. J. D., & Hiltunen, R. (2003). Antioxidant properties of extracts from selected culinary herbs and spices. Journal of the Science of Food and Agriculture, 83(9), 2334-2343. https://doi.org/10.1002/jsfa.1561
  4. Ghiselli, A., Nannelli, G., & Packer, L. (2000). Free radicals and antioxidants in the physiology of aging and age-related diseases. The Journal of Nutritional Biochemistry, 11(2), 24-38. https://doi.org/10.1016/S0955-2863(00)00144-2
  5. Gulcin, İ. (2020). Antioxidants and antioxidant methods: An updated overview. Archives of Toxicology, 94(3), 651-715.
  6. Goh, K. L., Chia, S. L., & Lim, P. H. (2016). Marine Collagen from Fish Skin: A Potential Source of Bioactive Peptides. Journal of Food Science, 81(5), 911-918. https://doi.org/10.1111/1750-3841.13135
  7. Halliwell, B., & Gutteridge, J. M. C. (2015). Free Radicals in Biology and Medicine (5th ed.). Oxford University Press.
  8. Issains, F.B., et al. (2019). Extraction of Collagen Type-I from Snakehead Fish Skin (Channa Striata) and Synthesis of Biopolymer for Wound Dressing. AIP Conference Proceedings, 2193(1), 20013. https://doi.org/10.1063/1.5139333
  9. Kharat, Z., et al. (2021). Chitosan/PEO Nanofibers Containing Calendula Officinalis Extract: Preparation, Characterization, In Vitro and In Vivo Evaluation for Wound Healing Applications. International Journal of Pharmaceutics, 609, 121132. https://doi.org/10.1016/j.ijpharm.2021.121132
  10. Li, Y., Zhang, J. J., Xu, D. P., Zhou, T., Zhou, Y., Li, S., & Li, H. B. (2013). Bioactivities and health benefits of wild fruits. International Journal of Molecular Sciences, 17(8), 1258.
  11. Liu, H., Zhang, Z., & Li, X. (2018). Denaturation Temperature and Structural Integrity of Collagen from Fish Scales: Implications for Biocompatible Cosmetics. Journal of Biochemistry and Molecular Biology, 15(3), 18-26. https://doi.org/10.1016/j.jbb.2018.01.015
  12. Menezes, E. A., Lima, A. A. D., & Santos, G. T. (2019). The effect of pH on the antioxidant properties of natural products: A systematic review. Journal of Food Science, 84(7), 1713-1722. https://doi.org/10.1111/1750-3841.14615
  13. Nikolaidis, N., et al. (2017). Denaturation of collagen and its effect on protein solubility and gel properties under acidic and high-temperature conditions. Food Biochemistry and Technology, 45(8), 47-56. https://doi.org/10.1016/j.foodchem.2017.03.015
  14. Prior, R. L., Wu, X., & Schaich, K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53(10), 4290-4302.
  15. Quevedo, A., et al. (2021). The impact of acidic conditions and temperature on collagen solubility and gel formation. Journal of Food Science and Technology, 54(5), 1229-1237. https://doi.org/10.1007/s11483-021-01618-3
  16. Rahman, M. M., Ahmed, T., & Hossain, M. M. (2019). Effects of Viscosity and pH on the Stability of Collagen Extracts for Pharmaceutical Applications. International Journal of Pharmaceutical Sciences, 14(4), 56-63. https://doi.org/10.1016/j.ijps.2018.09.019
  17. Setyaningsih, T., Supriyatna, P., & Santoso, S. (2020). The Antioxidant Properties of Collagen Extract from Fish Scales: A Review. Journal of Functional Foods, 68, 104535. https://doi.org/10.1016/j.jff.2020.104535
  18. Setyowiranti, M. F. ., & Darmaputra, I. G. N. . (2023). Snakehead Fish Extract in Increasing Skin Collagen & Fibroblasts. Jurnal Penelitian Pendidikan IPA, 9(11), 1189–1197. https://doi.org/10.29303/jppipa.v9i11.5496
  19. Yulistian, Y., Arfianti, I., & Sari, L. (2021). DPPH Assay for Antioxidant Activity: A Review of Techniques and Applications in Plant Extracts. Antioxidant Research, 8(2), 74-80. https://doi.org/10.1016/j.antres.2021.05.003